Gene interference using antisense oligodeoxynucleotides on whole chick embryos
Identifieur interne : 003743 ( Main/Exploration ); précédent : 003742; suivant : 003744Gene interference using antisense oligodeoxynucleotides on whole chick embryos
Auteurs : Jonathan Cooke [Royaume-Uni] ; Alison IsaacSource :
- Molecular Biotechnology [ 1073-6085 ] ; 2000-07-01.
English descriptors
- KwdEn :
Abstract
Abstract: Details are given of an advanced version of the ring method of chick embryo culture. This ensures good development from early blastoderm stages even when the culturing procedure is interrupted by the extended periods required for collecting matched embryo samples and for preparing antisense treatment. Antisense oligodeoxynucleotide treatment, in short-term incubation before return of blastoderms to their ring cultures, is then described. An alternative, roller-bottle, culture method for continued development after treatment is also described. Criteria for the validity and success of this gene interference method are given. While the toxt is meant to be of detailed prootioal help to these inexperienced in ombryo culture a preliminary reading, and familiarity with its sectional (Subheading) structure, is recommended before work is undertaken.
Url:
DOI: 10.1385/MB:15:3:259
Affiliations:
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Le document en format XML
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